In vitro and in vivo production of pectic enzymes, polygalacturonase, by seed-borne pathogen, fusarium moniliforme sheldon from seeds of rice (Oryzae sativa L) and its role in the disease of rice
Abstract
Possible in vitro and in vivo production of pectic enzyme polygalacturonase and pH on the activity of the enzyme were investigated. The results of the assay for the production of polygalacturonase (PG) by the pathogen showed that the activity was 142.9 RVU in vitro and 166.7 RVU, in vivo at temperature 25°C. The optimum activity of the enzyme in vitro was obtained at 25° C and 30°C, with the value of 142.9 RVU, and the least was at 20°C, 45°C and 50°C, with a value of 111.1 RVU. In vivo, the optimum activity was at 20oC, with a value of 166.7 RVU, while the least activity was 111.1 RVU at 50°C. The activity of PG in vitro was optimum at pH 5 and 6, with a value of 142.9 RVU each, while the least activity was obtained at pH 2, 9 and 10, with a value of 111.1 RVU. During the in vivo studies, the activity of PG was optimum at pH 6 with a value of 166.7 RVU and least at pH 2, 9 and 10, with the value of 111.1 RVU.Bio-Research Vol. 3(1) 2005: 9-13
Bio-Research journal is an open access journal, which means all its content is freely available without charge to the user or his/her institution.
You are free to share (that is, copy and redistribute the material in any medium or format) as long as you follow these licence terms:
Attribution (by)
You must give appropriate credit, provide a link to the license.
Non Commercial (nc)
You can copy, distribute, display, perform, and use this material for any purpose other than commercially (unless you get permission first). Non Commercial means not primarily intended for or directed towards commercial advantage or monetary compensation.
No Derivatives (nd)
If you remix, transform, or build upon the material, you may not distribute the modified material. But note that simply changing the format does not create a derivative.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the licence permits.
Notices
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.