In vitro and in vivo production of pectic enzymes, polygalacturonase, by seed-borne pathogen, fusarium moniliforme sheldon from seeds of rice (Oryzae sativa L) and its role in the disease of rice
Abstract
Possible in vitro and in vivo production of pectic enzyme polygalacturonase and pH on the activity of the enzyme were investigated. The results of the assay for the production of polygalacturonase (PG) by the pathogen showed that the activity was 142.9 RVU in vitro and 166.7 RVU, in vivo at temperature 25°C. The optimum activity of the enzyme in vitro was obtained at 25° C and 30°C, with the value of 142.9 RVU, and the least was at 20°C, 45°C and 50°C, with a value of 111.1 RVU. In vivo, the optimum activity was at 20oC, with a value of 166.7 RVU, while the least activity was 111.1 RVU at 50°C. The activity of PG in vitro was optimum at pH 5 and 6, with a value of 142.9 RVU each, while the least activity was obtained at pH 2, 9 and 10, with a value of 111.1 RVU. During the in vivo studies, the activity of PG was optimum at pH 6 with a value of 166.7 RVU and least at pH 2, 9 and 10, with the value of 111.1 RVU.Bio-Research Vol. 3(1) 2005: 9-13
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