Bioactivity of 2-amino-9-(4-oxoazetidin-2-yl) Nonanoic Acid from the Root Exudates of Cleome viscosa L
C12H22N2O3 [2-amino-9-(4-oxoazetidin-2-yl) nonanoic acid or 2-amino-9-β-lactum nonanoic acid(LNA), mol.wt. 242] has been isolated and purified from the root exudates of Cleome viscosa. Aqueous solution of this pure compound has been tested on bacteria (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and fungi (Aspergillus fumigatus, Aspergillus niger and Aspergillus tamarii). At a dosage of 500ppm and above, P. aeruginosa and S. aureus were totally inhibited while E. coli remained unaffected. On the other hand, growth of A. niger and A. tamarii was stimulated while there was no effect on A. fumigatus. In A. tamarii, however, a dosage of 500ppm exerted the minimal stimulatory effect while maximal stimulation occurred at 1000ppm and above whereas in A. niger maximal stimulation was observed at 500ppm but at 2000ppm there was no effect. In rice, it caused complete inhibition of germination from 1000ppm to 125ppm concentration while in the case of mustard seeds, complete inhibition was noticed only at 1000ppm concentration. At 500ppm, 17.22% and 66.01% inhibition on shoot and root length respectively was detected. In case of gram seeds, MFCV exerted complete inhibition of germination up to 500ppm concentration. At 250ppm, it showed 100% inhibition in shoot length and 50.41% inhibition in root length.
Keywords: Cleome viscosa, Allelochemical, Antimicrobial activity, Lactam nonanoic acid (LNA)
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