Damage mechanisms of pathogenic bacteria in drinking water during chlorine and solar disinfection

  • Seyram K. Sossou
  • Dotse K. Gbedenu
  • Yacouba Konate
  • Boukary Sawadogo
  • Yaovi Ameyapoh
  • Amadou H. Maiga
  • Naoyuki Funamizu
Keywords: Drinking water, pathogenic bacteria, E. coli, damage mechanisms, chlorine disinfection, solar disinfection

Abstract

This study aimed at elucidating the inactivation mechanisms of pathogenic bacteria in drinking water during chlorine and solar disinfection using a simple plating method. The well-known bacterial model Escherichia coli was used as pathogenic bacteria for the experiments. The damage mechanisms of E. coli were evaluated by simple plating method on selective, less selective and non-selective media. Results showed that, injured E. coli were detected at different levels during chlorine and solar disinfection. The use of selective media during water quality control showed effectively the destruction of E. coli during solar disinfection while the removal of E. coli during chlorine disinfection was not ensured. The damage of cell components and/or metabolic functions showed that there is a primary and mainly damage of E. coli during chorine and solar disinfection. Chlorination firstly and mainly damaged membrane cell followed by that of enzymatic functions and nucleic acid; while solar disinfection damaged mainly nucleic acid. The use of simple plating method in water quality control is limited by the choice of plating media depending on the disinfectant used. The understanding of the damage mechanisms of pathogenic bacteria cells during disinfection helps improve drinking water quality control and develops more effective disinfection strategies.

© 2016 International Formulae Group. All rights reserved.

Keywords: Drinking water, pathogenic bacteria, E. coli, damage mechanisms, chlorine disinfection, solar disinfection

Published
2016-09-12
Section
Articles

Journal Identifiers


eISSN: 1997-342X
print ISSN: 1991-8631