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Isolation and characterization of antibiotic susceptibility profile of Salmonella species isolated from abattoir environment

E. O. Igbinosa, A. Beshiru

Abstract


In this study we investigated the antibiogramic profile of Salmonella species isolated from abattoir environment. A total of 72 samples were collected from three different stations [station A (the drainage), station B (stagnant water in potholes and floors around the abattoir) and station C (water used for washing the meat slaughtered at the abattoir)] between January and June 2017. All samples were processed and analysed using standard culturebased and polymerase chain reaction (PCR) methods. The mean total heterotrophic bacteria population densities were: station A 2.0×1010 ± 0.02 CFU/ml; station B 1.6×1010 ± 0.10 CFU/ml and station C 3.9×109 ± 0.05 CFU/ml. The mean total salmonellae population density were 2.4×105 ± 0.14 CFU/ml,1.3×105 ± 0.15 CFU/ml, and 1.0×104 ± 0.06 CFU/ml for station A, station B and station C respectively. A total of 50 confirmed salmonellae, using PCR identification system, were subjected to antimicrobial profiling using the disc diffusion method. The resistance profile of the isolates revealed that 24 (48%) of the isolates were resistant to piperacillin, 50 (100%) to gentamycin, 50 (100%) were resistant to tetracycline, while 22 (44%) of the isolates were resistant to aztreonam. Multidrug resistance (MDR) profile of the isolates revealed that 22 (44%) of the isolates were resistant to piperacillin, gentamycin, tetracycline, and aztreonam belonging to four antimicrobial class. Our findings revealed that abattoir environments could be a potential reservoir of multi-antibiotic resistant Salmonella species.

Keywords: Abattoir, Salmonellae, Antibiogramic Profile, Multi-Drug Resistance, Effluent




http://dx.doi.org/10.4314/ijs.v19i2.19
AJOL African Journals Online