Vitamin C, Vitamin E, L-Arginine, Low dose, HbSS, Free radicals, Antioxidants.

Abstract

Backgroumd: Sexual disorders have been extensively studied in diabetic men while possible changes in the sexual function of diabetic women have only recently received attention. Mammals other than primates do not menstruate, thus their sexual cycle is termed “Estrous cycle”. The period of ‘heat’ (estrus), is the time of ovulation and corresponds to the only time which the sexual interest of the female mammal is aroused. The goal of this study was to assess the effects of alloxan-induced diabetes and insulin-resistant on hormonal pattern in diabetic female rats Methods: Virgin female Sprague-Dawley rats aged 6 weeks, weighing 90 – 100 g were randomly divided into 3 groups. Group 1 = Control group; fed on normal rat chow. Group 2 = Alloxan-diabetic group; at the 4th week received a single dose IV injection of alloxan monohydrate 40 mg/kg BW into the lateral tail vein. Group 3 = Insulin resistant group; fed ad libitum on a special diet containing 25% fructose mixed with 75% normal rat chow (w/w). Cervical dislocation was carried out on the animals in the three groups on the morning of specific phases of the estrous cycle and the LH, FSH, Estradiol, and Progesterone levels were determined using Enzyme-linked immunosorbent assay (ELISA) methods. On the morning of estrus phase, the oviducts were excised, viewed under the microscope and any ova found was counted. Results: The results of this study showed that both Alloxan-diabetes and insulin resistant diabetes distort the estrous cycle pattern, reduce significantly the “fertile period” and the ova released by acting on the pituitary-ovarian axis reducing the gonadotropins and the ovarian hormones. Conclusion: These data open a new field to study hormonal profile in diabetic female rats during each phase of the estrous cycle and also to investigate the hypothalamo-pituitary-ovarian axis in insulin-resistant diabetic female rats.in light of recent preferences for the use of natural medicines.