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Effect of varying concentrations of glucose and maltose on the Zygotic embryo propagation of Glycine max l. (Invitro)


U. C. Okafor

Abstract

The effect of varying concentrations of glucose and maltose was studied on the growth of Glycine max L. in vitro
using Murashige and Skoog media. Glucose and maltose concentrations were 2% and 4% each, while 0% (no
carbon source) served as control. Embryo explant of Glycine max L. in 4% glucose attained 80% growth from the
second day of inoculation followed by glucose at 2% level with 70% induction, whereas maltose at 4% and 2%
started also from the second day but with percentage induction of 60% each. Glucose based medium generally
exhibited the highest mean value of root and shoot length when compared to maltose based medium. Four percent
glucose has the highest mean, though not significantly different (P ≤ 0.05) from the control medium. The glucose
based medium shows greater efficiency both in shoot length and embryo growth induction than the maltose based
medium. However, glucose at 4% level is preferable to that of 2% level as observed from the growth parameter
analysis.


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print ISSN: 0300-368X