Cell Suspension Culture of Mucuna pruriens For Production and Improvement of L-3, 4-Dihydroxy Phenylalanine Concentration
Abstract
The production and improvement of L-3, 4-dihydroxy phenylalanine (L-Dopa) content in Mucuna pruriens was carried out through cell suspension culture. Thirty days old healthy, friable and soft calli derived from leaf and stem of two varieties of M. pruriens: (IIHR Selection 3 and Arka Dhanvantari) were used for the cell suspension in Murashige and Skoog’s liquid medium supplemented with IAA (1.0mg/L), NAA (1.5mg/L) and BAP (1.5mg/L) with treatment of elicitors: chitin and pectin (100, 150, and 200mg/L), precursor: L-tyrosine (5, 10, and 15mg/L) and ascorbic acid (250mg/L). The highest percentage of packed cell volume (PCV) (42.4 ± 0.1) was observed in leaf-derived callus of AD variety with treatment L4: MS + NAA (1.5mg/L) + Pectin (100mg/L) + ascorbic acid (250mg/L). From the HPLC analysis, treatment T3c: MS + IAA (1.0mg/L) + L-tyrosine (15mg/L) + ascorbic acid (250mg/L) recorded highest peak area percentage of 31.19 at retention time (RT) of 2.36 against control (1.39) at 2.35 RT. L-Dopa concentration was observed to increase with increase in elicitor and precursor treatment. This shows that L-Dopa can be produced from natural source in desired concentration through cell suspension culture.
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