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Bambara groundnut (Vigna subterranea [L.] Verdc.) is a seed legume native to West Africa with significant agronomic, nutritional, economic and socio-cultural potential. In Burkina Faso, its cropping faces enormous constraints from poor soil fertility and poor nutrient management practices. This study aimed to evaluate the effects of different doses of organic manure on Bambara groundnut production. Experiment was conducted in experiment site of Tenkodogo University Center from July to October 2021. The treatments consisted of 0; 2; 3; 4; 5 t ha-1of cow dung. Experimental design consisted of the trial following a completely randomized block design with four replications. Crop management consisted of a flat plowing, direct application of organic manure, sowing distances of 0.40 m x 0.20 m and ridging at 49 days after sowing. Compared to the control, results showed that application of 5 and 4 t ha-1 of organic manure significantly increased plant heights of Bambara groundnut by 19 and 20% (P < 0.001); 4 t ha-1 of organic manure treatment increased plant widths by 19% (P < 0.001); 5 and 4 t ha-1 of organic manure significantly increased the Bambara groundnut haulm yields by 37 and 30 % (P < 0.05) respectively. Significant improvement by 25% (P < 0.05) of nodules dry weight was observed under 2 t ha-1 of organic manure application. However, no significant correlations between plant biomass and numbers or weight of nodules was recorded. Thus, application of 2 t ha-1 of organic manure should be able necessary for Bambara groundnut nodulation and 4 or 5 t ha-1 of organic manure for high growth and haulm production of Bambara groundnut. Further investigations are needed to better understand the combined effects of soils and organic fertilizer interactions on Bambara groundnut productivity and soil fertility.