Study on the Solid Phase Extraction and Spectrophotometric Determination of Mercury in Water and Biological Samples with 5-(p-Aminobenzylidene)-thiorhodanine
This paper presents the synthesis and application of 5-(p-aminobenzylidene)-thiorhodanine (ABTR) as a new chromogenic reagent for the determination of mercury. Based on the rapid reaction of mercury(II) with ABTR and the solid phase extraction of the coloured chelate with a C18 disk, a highly sensitive, selective and rapid method for the determination of mercury has been developed. In the presence of pH 3.5 sodium acetate-acetic acid buffer solution and emulsifier-OP medium, ABTR reacted with mercury(II) to form a red chelate in a molar ratio 1:2 (mercury to ABTR). This chelate was enriched by solid phase extraction with a C18 disk and eluted from the disk with dimethyl formamide (DMF). An enrichment factor of 50 was achieved. The molar absorptivity of the chelate in DMF was found to be 1.21 × 105 L mol–1 cm–1 at 555 nm. Beer’s law was obeyed in the range of 0.01~ 3 μgmL–10/. The relative standard deviation for eleven replicates with a concentration of 0.01 μgmL–1 was 1.98%. This method was applied with good results to the determination of mercury in water and biological samples.
Keywords: Mercury, solid phase extraction, spectrophotometry, 5-(p-aminobenzylidene)-thiorhodanine.
Copyright for articles published in this journal is retained by the journal.
SAJChem applies the Creative Commons Attribution (CC BY) license to manuscripts we publish. This license was developed to facilitate open access – namely, free immediate access to, and unrestricted reuse of, original works of all types. Under this license, authors agree to make articles legally available for reuse, without permission or fees, for virtually any purpose. Anyone may copy, distribute or reuse these articles, as long as the author and original source are properly cited.