Spectrophotometric and Voltammetric Studies on the Interaction of Heparin with Crystal Violet and its Analytical Application
The interactions of heparin with crystal violet were studied by absorption spectrophotometry and voltammetry in pH 3.0 Britton-Robinson (B-R) buffer solution. Heparin, which is negatively charged, can easily bind to the positively charged crystal violet to forma supramolecular ion-association complex. Owing to the formation of the new complex, the maximum absorption wavelength of crystal violet at 592nmdecreased and two new absorption peaks appeared at 510nmand 363nmafter the addition of heparin. The oxidation peak current of crystal violet on glassy carbon electrode (GCE) at +0.84V(vs. SCE) also changed correspondingly without change of the peak potential, which also indicated that the binding reaction had taken place. Under the selected conditions a new spectrophotometric analytical method was established for heparin with the linear range between 0.10–4.0 mg L–1) with a linear regression equation as ΔA = –0.002 + 0.227C (mg L–1), (n = 8, γ = 0.997). The relative standard deviation for eleven parallel determinations of 0.40mgL–1) heparin was 1.69% and the detection limit (3σ) was 0.09mgL–1). Thisnew method was further applied to the determination of heparin sodium injection samples with satisfactory results.
KEYWORDS: Heparin, crystal violet, spectrophotometry, voltammetry, ion-association complex.
Copyright for articles published in this journal is retained by the journal.
SAJChem applies the Creative Commons Attribution (CC BY) license to manuscripts we publish. This license was developed to facilitate open access – namely, free immediate access to, and unrestricted reuse of, original works of all types. Under this license, authors agree to make articles legally available for reuse, without permission or fees, for virtually any purpose. Anyone may copy, distribute or reuse these articles, as long as the author and original source are properly cited.