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Published:
Oct 6, 2015Keywords:
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Supercritical Fluid Extraction of Seed Oil from Chinese Licorice <i>Glycyrrhiza uralensis</i> Fisch.): Chemical Composition and Antibacterial Activity
Abstract
Seed oil from Glycyrrhiza uralensis Fisch. was extracted by supercritical fluid (CO2) extraction. The oil was analysed by GC-MS after methylation. Compounds were identified according to their mass spectra (EI, 70 eV) by comparison with authentic reference substances and literature data. Five fatty acids were identified, with linoleic acid (24.3%) and α-linolenic acid (25.51%) being the main constituents. The effects of extraction time, pressure, temperature and CO2 flow rate on seed oil yield were investigated. Results showed that, under dynamic extraction time of 1.5 h, pressure 30MPa, temperature 50 °C and CO2 flux of 10 kg h–1, the oil yield was 2.09% (m/m). The method was efficient for the extraction of this oil. Antibacterial activity of the oil was checked against Gram-negative and Gram-positive strains, evaluated by investigation of MIC and MBC. The seed oil of Glycyrrhiza uralensis did not exhibit an antibacterial effect against the tested strains in our test system.
KEYWORDS: Fatty acid, methylation, GC-MS, SCFE-CO2, Glycyrrhiza uralensis Fisch., antibacterial activity.
