Detection of Helicobacter pylori Reactive IgA and IgG Antibodies Using Enzyme Linked Immunsorbent Assay (ELISA)
AbstractBackground: Helicobacter pylori (H. pylori) infection is usually acquired
during the early years of life and persists for several years. Recent investigations pointed to a potential role of H. pylori infection of the upper gastrointestinal tract as a possible causative agent in chronic urticaria (CU).
Objectives: This study is aiming at evaluating the relationship between H. pylori and chronic urticaria in Sudanese patients.
Methods and patients: Fifty patients with idiopathic CU, twenty five of
other skin disorder and ten healthy controls were enrolled estimated for serum H.pylori Immunoglobulin G (IgG) and A (IgA) antibodies levels using Enzyme Linked Immunsorbent Assay (ELISA). All the results were analyzed using the Microsoft Office Excel (Microsoft Office Excel for windows; 2003) and SPSS (SPSS for windows 17).
Results and discussion: The IgG and IgA levels of control group were
significantly lower compared with both patients with chronic urticaria (P = 0.001 for IgG) (P = 0.001 for IgA) and patients with other skin disorders (P = 0.011 for IgG) (P = 0.006 for IgA). Receiver Operating Characteristic curve (ROC curve) analysis revealed that the area under ROC curves of IgA is higher compared to IgG (0.80 0Vs 0.890). In conclusion the current study revealed that patients with H. pylori infection have an increased tendency to develop urticaria. H. pylori reactive IgA antibodies correlate best with the infection.