Article Sidebar
Published:
Mar 15, 2019Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
<i>In-vitro</i> studies on acetylcholinesterase and butyrylcholinesterase inhibitory potentials of aerial parts of <i>Vernonia oligocephala</i> (Asteraceae)
Abstract
Methods: Crude methanol extract of V. oligocephala leaves and its fractions including n-hexane, dichloromethane (DCM), ethyl acetate and n-butanol were investigated for their inhibitory potentials against cholinesterases enzymes employing standard ELISA microtiter plate reader assay.
Results: All the extracts were moderately active against both the tested enzymes. The ethyl acetate and n-hexane fractions showed highest inhibition against acetylcholinesterase enzyme with IC50 values of 128.81±0.44 μg/mL and 200.51±0.22 μg/mL, respectively. In case of butrylcholinesterase, ethyl acetate (IC50; 145.71±0.19μg/mL) and DCM (IC50; 269.31±0.45 μg/mL) fractions were the most active. All other fractions including crude methanol extract exhibited least inhibition. Overall, ethyl acetate fraction was the most active against both enzymes.
Conclusion: Results indicate the promising potential of V.oligocephala as source of new potential compounds for management of Alzheimer’s disease. However, further studies to isolate and identify the potential bioactive components are needed.
Keywords: Vernonia oligocephala, Acetylcholinesterase, Butyrylcholinesterase, Alzheimer’s disease
