Morphological and Molecular Identification of Pratylenchus goodeyi Associated with Banana in Tanzania

  • Doreen Mgonja Tanzania Agricultural Research Institute, Mikocheni, P. O. Box 6226, Dar es Salaam, Tanzania
  • Gladness Temu Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dares Salaam, P. O. Box 35179, Dares Salaam, Tanzania
  • Magreth Mziray Tanzania Agricultural Research Institute, Kibaha, P. O. Box 30031, Kibaha, Tanzania
  • Beatrice Kashando Tanzania Agricultural Research Institute, Kibaha, P. O. Box 30031, Kibaha, Tanzania
  • Ambilikile Mwenisongole Tanzania Agricultural Research Institute, Kibaha, P. O. Box 30031, Kibaha, Tanzania
  • Minza Masunga Tanzania Agricultural Research Institute, Kibaha, P. O. Box 30031, Kibaha, Tanzania
  • Sylvester Lyantagaye Mbeya College of Health and Allied Sciences, University of Dar es Salaam, P. O. Box 608, Mbeya, Tanzania
  • Nessie Luambano Tanzania Agricultural Research Institute, Kibaha, P. O. Box 30031, Kibaha, Tanzania
Keywords: Genetic variation, Morphology, Musa sp., Root lesion nematode,

Abstract

Banana (Musa sp. [L]) is an important staple food and cash crop for about 30 percent of the total population in Tanzania. Root lesion nematodes, particularly Pratylenchus goodeyi, are among the major pests affecting banana production in Tanzania. A combination of morphological and molecular approaches is required for accurate identification of nematodes. In this study, 80 P. goodeyi isolates affecting bananas in Tanzania were identified. Sequence analysis of part of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) regions of ribosomal DNA (rDNA) confirmed the morphological identification of P. goodeyi. DNA sequences were analysed using Geneious version 11.0 software and phylogenetic relationship was constructed using Bayesian inference (BI) method. Morphological identification shows that P. goodeyi in this study resembles P. goodeyi identified from other parts of the world and their sequences clustered closely (98.6 – 99.9 % similarity) with other P. goodeyi sequences in the GenBank. The results obtained from this study will provide an insight on the presence of harmful root lesion nematodes in Tanzanian bananas, contributing to further studies aimed at developing efficient nematode management strategies in Tanzania.

Keywords: Genetic variation; Morphology; Musa sp.; Root lesion nematode;

 

Published
2019-07-16
Section
Articles

Journal Identifiers


eISSN: 2507-7961
print ISSN: 0856-1761