In vitro effect of Aqueouscalotropis procera root extract on ammonium sulphate precipitated liver marker enzymes of albino rats
The in vitro effect of aqueous root extract of C. procera on liver marker enzymes; alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) of albino rats was evaluated. ALT was precipitated at 40% ammonium sulphate saturation whilst AST and ALP were precipitated at 35 % saturation from rat liver homogenate. The enzymes were assayed at varying concentrations (mg/ml) of the extract (0.00, 0.05, 0.10, 0.15, 0.20, 0.25, 0.30, 0.35, 0.40, 0.45. 0.50) at 37oC. At 0.00µmg/ml extract; ALT, AST and ALP activities of 5.91 ~ 10-4, 2.70 ~ 10-4 and 3. 74 ~ 10-5 µmol/min respectively. Upon incubation with extract, the enzymes had respective mean activities of 6.38 } 0.35 ~ 10-4, 4.07 } 0.62 ~ 10-4and 2.80 } 0.44 ~ 10-5µmol/min. The activities of ALT and AST were significantly increased (P < 0.05) in presence of C. procera extract with significant decrease (P < 0.05) in the activity of ALP. It indicates that the aqueous root extract of C. procera activated ALT and AST and inhibited ALP in vitro.
Keywords: in vitro, C. procera,liver marker enzymes, ammonium sulphate