The effect of genotype and 2, 4-D on callogenesis was investigated in sugarcane (Saccharum spp.) hybrid cultivars; SP726180, B47419, M1176/77 and M2119/88. To evaluate the response of the genotypes to callus induction and embryogenic callus production, leaf base explants were cultured on Murashige and Skoog (MS) basal medium supplemented with 2.5, 3.0, 3.5, or 4.0mg/L) 2,4-D for 8wks. While, the number of responding explants was found to generally increase with the increase in 2,4-D concentration from 2.5 to 4.0mg/L, embryogenic callus producation was highest on media
supplemented with 3.0 and 2.5mg/L. There were no significant differences in the number of responding explants and embryogenic callus production among B47419, M1176/77 and M2119/88, these genotypes however exhibited a significantly higher response when compared to SP726180 indicating their high propensity to in vitro callogenesis.