Hepatoprotective and Anticancer Potentials of<i>Moringa oleifera</i> and <i>Musa sapientum> Extracts against Cadmium Chloride Induced Hepatotoxicity in Rats

Abstract

Anticancer potential of MO11 (fractionated from Moringa oleifera leaves) and MS06 (fractionated from Musa sapientum suckers) against cadmium chloride induced hepatotoxicity, demyelination, carcinogenesis, and metastasis is reported. The activity was evaluated for 17 days in 24 adult male Wistar rats randomly divided into six groups (n=4). The baseline control Group 1 received normal saline only for the entire study period. Groups 2, 3, 4 and 6 received single CdCl-dose on Day 1. Group 2 (negative control) received no further treatment, while Groups 3, 4 and 6 were treated with plant extracts MO11, MO11+MS06, and doxorubicin (positive control), respectively, on Days 1-17. Group 5 received olive oil vehicle only for the 17 days. Levels of neurotransmitters (dopamine and glutamate), and biomarkers of myelination (myelin basic protein, MBP), drug metabolism and carcinogenesis (cytochrome p450), apoptosis (caspase-3 and p53), and angiogenesis (soluble vascular endothelial growth factor receptor, sVEGFR) in liver homogenates were determined using enzyme-linked immunosorbent assay. The data were statistically analysed using Mann-Whitney U test with p ≤ 0.05. The MO11, MO11+MS06, and doxorubicin upregulated dopamine, glutamate, and cytochrome p450, but downregulated MBP, caspase-3, p53 and sVEGFR in Groups 3, 4 and 6, compared with Group 2, implying the hepatoprotective, re-myelination, and anticancer potential of the studied plant fractions.