Plasmid Borne Resistance in Klebsiella Isolates from Kenyatta National Hospital, Nairobi, Kenya

  • JM Bururia
  • PN Kinyanjui
  • PG Waiyaki
  • SM Kariuki
  • PN Karimi
Keywords: Plasmid, resistance, conjugation, transformation.

Abstract

Eighty six Klebsiella isolates from Kenyatta National Hospital and the Centre for Microbiology, Kenya Medical Research Institute, Nairobi were screened for
resistance to commonly prescribed antimicrobial agents and for their plasmid
content. Plasmids were transferred into Esherichia coli K-12 and resulting
transconjugants screened for resistance to the antimicrobial agents used on
Klebsiella donors and for their plasmid content. Plasmids from the Klebsiella
isolates were also transformed into Eschericia coli and transformants analyzed
for resistance and plasmid content. Endonuclease restriction mapping was done to characterize the plasmids from Klebsiella isolates and their Eschericia coli transformants. Resistance was found to be plasmid borne and transmissible.
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eISSN: 1026-552X