Liquid chromatographic analysis of phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column

  • B K Amugune Department of Pharmaceutical Chemistry, School of Pharmacy, University of Nairobi, P.O. Box 19676- 00202, Nairobi, Kenya.
  • G N Thoithi
  • I O Kibwage
Keywords: Liquid chromatography, co-polymer column, phenobarbitone, ethosuximide, phenytoin, carbamazepine.

Abstract



A liquid chromatographic method for the simultaneous assay of four anticonvulsant drugs, phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column is described. The method was developed by the systematic study of different types of co-polymer materials, type and concentration of organic modifiers, buffer pH and concentration and column temperature. A PLRP-S 100 Å 8 µm column maintained at 60 oC and a mobile phase consisting of acetonitrile-tert-butanol-phosphate buffer (pH 7.6, 0.2 M)-water (25:5:10:60, v/v) were used. The flow rate was 1 ml/min with ultraviolet detection at 220 nm. The method has been validated and used for the analysis of raw materials, finished products and dissolution studies of the drugs.

Keywords: Liquid chromatography, co-polymer column, phenobarbitone, ethosuximide, phenytoin, carbamazepine.

The East and Central African Journal of Pharmaceutical Sciences Vol. 8 (3) 2005: pp 19-25
Published
2007-10-24
Section
Articles

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eISSN: 1026-552X