Effects of adiponectin on markers of endothelial activation and markers of inflammation in human coronary artery endothelial cells

  • N. A. Rosman
  • G.R.A. Froemming
  • O. Effat
  • H. Nawawi
  • H.J. Singh
Keywords: HCAEC, Adiponectin, ICAM-1, E-selectin, IL-6, PAI-1, COX-2, NFkB.

Abstract

This study investigates the effect of adiponectin on endothelial activation and inflammatory marker secretion by human coronary artery endothelial cells (HCEAC) in vitro.
Methodology: HCAEC at the seventh passage were divided into two groups and incubated or 24 hours at 37° C and 5% CO2 as follows: Control, and adiponectin-treated (30 μg/ml adiponectin) groups. Supernatants were analysed for ICAM-1, E-selectin, PAI-1 and IL-6 and COX-2 using ELISA. RT-PCR was used to analyse gene expression of ICAM-1, E-selectin, PAI-, IL-6, COX-2, NFkBp50 and NFkBp65. Data were analysed using independent t-test.
Results: ICAM-1 and E-selectin level was significantly higher in leptin-adiponectin-treated groups (P<0.01). Endothelial activation marker protein level have no significant difference in adiponectin group when compared to control group.  mRNA expression showed significant increase in adiponectin group as compared to control. PAI-1 and COX-2 showed no significant increase in the level of protein in adiponectin group but IL-6 showed significant increased in the protein level (P<0.001) while mRNA expression of PAI-1 (P<0.05), COX-2 (P<0.01) and IL-6 (P<0.001) showed significant increase in treated group as compared to control. mRNA expression also showed significant increase in both the NFkBp50 and NFkBp65 signalling pathway.
Conclusion: Adiponectin increases the secretion of IL-6 from HCEAC. This adipokines
might have a significant role in the inflammatory and pro-atherogenic state of obesity.

Keywords: HCAEC, Adiponectin, ICAM-1, E-selectin, IL-6, PAI-1, COX-2, NFkB.

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eISSN: 1112-9867