The objectives of this study were to establish whether demographic characteristics, interpersonal relationships, terms of work and work life balance influence the retention of academic staff at Makerere University and Kyambogo University. The study followed a cross-sectional survey design. Data were collected using a self-administered questionnaire from 298 academic staff of the two universities. The respondents were selected using stratified random sampling. The data were analysed using student t test, ANOVA and simple linear regression. The findings were that gender, terms of work and work life balance significantly influence retention. Marital status, age, work experience, level of education and interpersonal relationships were also found to influence retention albeit insignificantly.