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Nigerian Journal of Technology

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Identification of the Bacterial Community Responsible for Decontaminating Eleme Petrochemical Industrial Effluent Using 16s RDNA PCR Denaturing Gradient Gel Electrophoresis

CU Ajuzie, EI Atuanya

Abstract


Identification of bacteria community responsible for decontaminating Eleme petrochemical industrial effluent using 16S PCR denaturing gradient gel electrophoresis (DGGE) was determined. Gene profiles were determined by extracting DNA from bacterial isolates and amplified by polymerase chain reaction (PCR) using 16S rDNA eubacterial primers with a GC clamp. PCR amplified DNA was separated by Denaturing Gradient Gel Electrophoresis (DGGE) analysis. Comparative evaluation of primers for PCR amplification of DNA revealed that V6V8F and V6V8R primers were most suitable for amplification DNA. Denaturing gradient gel ratio of 20% : 80% was most suitable for differentiation of bacterial profiles. Sequencing and phylogenetic analysis of DGGE products were successful displaying 88% -100% similarity and homology to Roseococcus sp. LW5(FM956480.1). Dendograms and similarity matrix revealed 81.5% -100% from sampling points.

http://dx.doi.org/10.4314/njt.v33i4.6




http://dx.doi.org/10.4314/njt.v33i4.6
AJOL African Journals Online