Potential use of Mangifera indica seed kernel and Citrus aurantiifolia seed in water disinfection
High cost of chemical disinfectants used in water treatment is one of the most crucial factor rendering water treatment expensive, not only which, the health risk posed by these chemicals are also of concern to many researchers. Hence, exploration of natural products as alternative disinfectant is a growing research area. In this study, sodium chloride extract of mangnifera indica (Duncan mango) seed kernel and citrus aurantiifolia (key lime) seed were investigated using agar well diffusion and micro broth dilution methods for their antibacterial activity against some enteric pathogenic organisms; Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212); Escherichia coli (ATCC25922), Klebsiella spp. (clinical strain), Pseudomonas spp. (ATCC4853), and Salmonella typhi (clinical strain). Results revealed that the extracts have antimicrobial activity against the test organisms. In agar well diffusion method, the extracts were most effective at concentration 100mg/ml. In the broth dilution method, the extracts were bacteriostatic at lower concentration and bactericidal at higher concentration. Key lime seed extract was found to have the least minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on Escherichia coli compared with chlorine, hence it was more potent on Escherichia coli than chlorine in liquid broth medium. The antimicrobial activity of the extracts against the test organisms were comparable to that of chlorine. The study revealed that Duncan mango seed kernel and key lime seed extracts have antimicrobial properties which can be made more effective as that of chlorine, thus this will be relevant in water disinfection and will improved water treatment in poor developing countries. It is concluded that the extracts have potential use in water disinfection.
Keywords: Disinfection, Mangifera indica seed kernel, Citrus aurantiifolia seed, Chlorine, Seed extract