First report of Chryseobacterium indologenes as causal agent for crown rot of papaya (Carica papaya L.) in peninsular Malaysia
Bacterial strains were isolated from papaya plants showing the crown rot symptoms in peninsular Malaysia. Greasy and water-soaked lesions were observed on petiole axis, young stems and buds of the plants. Bacteria were then identified using the Biolog system showed that the bacterium was Chryseobacterium indolegenes with a similarity (SIM) index of between 0.5 and 0.74 at 24 h of incubation followed by standard morphological and biochemical tests. The isolates were then confirmed by Polymerase Chain Reaction (PCR) and sequencing of the 16S rRNA gene and was successfully identified as C. indologenes with a 100% sequence similarity with reference strain (C. indolegenes strain LMG 8337; GenBank Acc. No: NR_042507.1). C indolegenes was consistently isolated from diseased papaya plants and the pathogenicity was confirmed by Koch’s postulate.
Keywords: papaya crown rot; Chryseobacterium indologenes